DMEM/F-12 Cell Culture Medium
KZT 14634
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DMEM/F-12 is widely used for supporting the growth of many different mammalian cells
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Информация о продукте
| Date First Available | January 31, 2023 |
| Manufacturer | Hygia Reagents |
| Brand Name | Hygia Reagents |
| UPC | 755457565786 |
| Part Number | HCM-DMEM |
| Unit Count | 1.00 Count |
| Included Components | 1 bottle |
| Item Weight | 2 lbs (910 grams) |
Who Should Buy?
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Cell Biologists
Ideal for researchers studying various cell types in vitro, supporting diverse growth and differentiation protocols.
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Tissue Engineering
Suitable for applications in tissue engineering where cell growth and maintenance are critical for developing artificial tissues.
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Pharmaceutical Research
Beneficial for drug discovery and testing, providing a stable environment for human and animal cells during experimentation.
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Plant Cell Cultures
Not suitable for plant cell cultures which require specific nutrients and growth factors not found in DMEM/F-12.
ОПИСАНИЕ ТОВАРА
DMEM/F-12 Cell Culture Medium
Вопросы и ответы клиентов
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вопрос:
What is DMEMF-12 Cell Culture Medium used for?
отвечать: DMEMF-12 Cell Culture Medium is primarily used for the growth and maintenance of mammalian cells in laboratory settings. It is specifically formulated to support the nutritional needs of fast-growing cells, providing essential amino acids, vitamins, and minerals. Researchers commonly utilize this medium in experiments involving cell proliferation, genetic engineering, and drug development, ensuring optimal conditions for cell viability and functionality. -
вопрос:
How do you prepare DMEMF-12 Cell Culture Medium?
отвечать: Preparing DMEMF-12 Cell Culture Medium involves reconstituting the powdered form with sterile distilled water or a suitable buffer. It is important to adjust the pH to around 7.2-7.4, which provides the necessary environment for cell growth. Additionally, supplementing the medium with fetal bovine serum or other growth factors enhances its effectiveness. This preparation is crucial as it ensures that the cells receive the right nutrients and maintain their health during experiments. -
вопрос:
Can DMEMF-12 be used for primary cell cultures?
отвечать: Yes, DMEMF-12 Cell Culture Medium can be utilized for primary cell cultures, particularly for a variety of mammalian cells. Its formulation is designed to cater to the growth requirements of both established cell lines and primary cells. Its versatility makes it ideal for researchers conducting studies on tissue regeneration, cellular response, and metabolic processes in a closer-to-native environment. -
вопрос:
What makes DMEMF-12 different from other culture media?
отвечать: DMEMF-12 differentiates itself from other culture media by its unique blend of nutrients tailored for fast-growing cells. Its composition allows for better cell attachment, proliferation, and metabolic activity compared to standard DMEM. Researchers select DMEMF-12 for high-cell-density applications, ensuring improved experimental outcomes in studies related to cancer research, regenerative medicine, and pharmacological testing. -
вопрос:
Is DMEMF-12 suitable for serum-free culture?
отвечать: DMEMF-12 can be adapted for serum-free culture by supplementing it with specific growth factors and hormones tailored to the cell line being used. This serum-free approach minimizes variability and allows for a more defined environment. Many researchers prefer serum-free conditions for regulatory and consistency reasons, especially when conducting assays or scales relevant to biotech applications. -
вопрос:
How should DMEMF-12 Cell Culture Medium be stored?
отвечать: DMEMF-12 Cell Culture Medium should be stored in a cool, dry place away from direct sunlight. Once prepared, it is best to store the medium at 2-8 degrees Celsius for short-term use or freeze it for long-term storage. Proper storage is essential to maintain its integrity and effectiveness, ensuring that the cells receive the necessary nutrients and conditions for optimal growth. -
вопрос:
What cell lines are commonly grown in DMEMF-12?
отвечать: Commonly used cell lines such as HEK293, CHO, and various cancer cell lines thrive in DMEMF-12 Cell Culture Medium. These cell lines are often employed in genetic, pharmaceutical, and toxicological studies. Researchers choose DMEMF-12 to support robust growth and high cell yield, which is crucial for experiments requiring significant cell quantities for analysis. -
вопрос:
Can DMEMF-12 be modified or supplemented?
отвечать: DMEMF-12 can be modified or supplemented to better suit specific cell growth needs. Researchers might add components such as L-glutamine, non-essential amino acids, or specialized growth factors depending on the cell type. Customization allows for greater control over growth conditions, enabling researchers to optimize their cultures for experiments related to gene expression, cell signaling, and drug response. -
вопрос:
What are the signs of spoiled DMEMF-12 Medium?
отвечать: Signs of spoiled DMEMF-12 Medium include changes in color, presence of precipitate, or an unusual odor. Additionally, if cell cultures show poor growth or abnormal morphology after media change, it may indicate spoilage. For consistent and reproducible results, it is imperative to routinely check the medium for these indicators and replace it when necessary to maintain cell health and experimental accuracy. -
вопрос:
Where can I buy DMEMF-12 Cell Culture Medium in Kazakhstan?
отвечать: You can purchase DMEMF-12 Cell Culture Medium through Ubuy in Kazakhstan. Ubuy offers a wide range of laboratory supplies and ensures that you can find the specific formulations you need for your research. With various products available, Ubuy serves as a convenient platform for researchers looking to acquire high-quality cell culture media.
Hygia Reagents Analytical Reagents Editorial Review
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KZT 14634
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Особенности и преимущества
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12
- It contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture.
- DMEM/F-12 contains no proteins, lipids, or growth factors which requires additional supplementation.
- Hygia’s cell culture media products are screened through strict quality control testing.
- Manufactured at a cGMP-compliant facility using sterilized water.
- Each lot of DMEM/F-12 is subjected to multiple tests to ensure the highest quality.
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